Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: IQGAP1 and IQGAP3 are critical host factors for Marburg virus replication, nucleocapsid transport, and cell-to-cell spread

doi: 10.1007/s00018-025-06047-3

Figure Lengend Snippet: Impact of IQGAP knockout on intracellular transport dynamics of MARV nucleocapsids. Huh-7 IQGAP KO and control cells were infected with a recombinant MARV encoding VP30-GFP to visualize nucleocapsids. Live-cell imaging was performed at 48 h h p.i. using a Leica DMI6000 microscope. Time-lapse image sequences were acquired at 500 ms intervals over a period of 100 s. For each clone, two independent experiments were conducted, capturing a minimum of 14 cells and 27,000 trajectories. Particle tracking was carried out using the IMARIS Spots™ function. A . Representative nucleocapsid trajectories are shown with color coding from cyan to magenta, indicating increasing velocity from 0 to 1 μm/s. White arrows highlight fast-moving nucleocapsids at the cell periphery (magenta, approximately 1 μm/s). The amount (n) of trajectories with a displacement (d) of more than 5 μm is indicated per shown example for each cell clone. B. Quantitative analysis of nucleocapsid transport: bar graph with plotted single data points displays the mean values ± standard deviation in percentage of trajectories with displacements greater than 5 μm. Statistical significance was assessed using Tukey´s multiple comparisons test with an adjusted p-value defined as follows p ≤ 0.01 (**), p ≤ 0,001(***), p ≤ 0,0001 (****). Non-significant differences are not indicated

Article Snippet: NCs were identified using the Spots detection algorithm in IMARIS (Bitplane, Oxford Instruments), with the estimated spot diameter set to 1 μm.

Techniques: Knock-Out, Control, Infection, Recombinant, Live Cell Imaging, Microscopy, Standard Deviation